Advancing best practices in cryogenic cold chain solutions.
Advancing best practices in cryogenic cold chain solutions

Sharing fundamental research, education, discussion and best practices for cryogenic cold chain management, biobanking, glass transition; biosample storage, preparation, planning and recovery and associated issues.


John Fink

John Fink is marketing manager for cryogenic solutions at Brooks Life Science Systems, the global leader in automated cold-chain sample management for drug discovery and biostorage applications and a division of Brooks Automation, Inc. if you have any questions or ideas for future blog posts.

Sample warming research: Warming rates may be surprising

May 27, 2015

Good biological cryogenic practices tell us to store as cold as possible, minimize freeze-thaw cycles and always protect innocents from warming above Tg (the glass transition temperature, -135°C for H2O). But just how quickly do samples warm? Are there variables that speed or slow warming?

Dr. Matteo Salvetti, Sr. Product Engineer, presented data at ISBER 2015 which discussed the thermal excursions of different types of vials within different types of cryoboxes. The results are surprising. For example, samples can warm as quickly as 70°C/minute and a sample can warm from -180°C to -135°C (Tg) in less than 40s. A vial’s location and its surrounding vials have a huge effect on its warming rate.

And did you know that when using a cryobox with holes in the bottom, its samples will warm faster when placed from -180°C onto dry ice than when placed in an ambient environment? Quite counterintuitive, but likely due to increased convection from the sublimation of the dry ice.

Download Dr. Salvetti’s poster: “Bio-Samples Thermal Protection.”