Surprising new warming research – at the vial level
June 9, 2015
It’s important to understand sample warming at all levels of your workflow and with all types of consumables to truly manage your cold chain. At ISCT in Las Vegas, Dr. Salvetti presented a poster on thermal excursion research at the vial level — which nicely complements his ISBER cryobox warming research poster.
In every workflow, individual vials are accessed (picked) and when they are separated from their cryo surroundings, they warm extremely quickly: From -173°C to Tg of water (-135°C) in as few as 9 seconds! Additionally, in every experiment tested here, the vials warm faster to -90°C on dry ice than in ambient. This phenomenon warrants a separate conversation (and future posting) which I plan to have with Dr. Salvetti to dig deeper into why this is happening.
In summary, understanding how fast vials will cross glass transition (Tg) allow scientists to create SOPs to ensure valuable samples stay well frozen (or should I say “glassy”). Understanding the individual variables effecting warming is also important. Obviously, the temperature delta is most critical, but the fill volume and touching the vial lid has minimal increased warming effect.
Download the ISCT poster which showcases all this data: “Thermal excursions of cryogenically frozen vials.”