Advancing best practices in cryogenic cold chain solutions.
Advancing best practices in cryogenic cold chain solutions

Sharing fundamental research, education, discussion and best practices for cryogenic cold chain management, biobanking, glass transition; biosample storage, preparation, planning and recovery and associated issues.

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John Fink

John Fink is marketing manager for cryogenic solutions at Brooks Life Science Systems, the global leader in automated cold-chain sample management for drug discovery and biostorage applications and a division of Brooks Automation, Inc. if you have any questions or ideas for future blog posts.

Cryopreservation Best Practices for CAR-T cell Storage

October 25, 2017

For over a year we stored Jurkat CAR-T cells in four different conditions to understand what temperature and cryoprotectant media performs best.  The two temperature conditions were -80°C and -190°C and the two medias were a home-brew cocktail of FBS/DMSO 95%/5% and cGMP manufactured CryoStor5.  At ISBER earlier this year we presented the results [download poster here].

In summary, the highest performing group was the Jurkat T-cells prepared with CryoStor5 media and stored at -190°C.  Their post-thaw viability and metabolic activity after 410 days in storage was nearly identical to the non-shipped control.  The group stored at -190°C with home-brew media had reduced viability and both groups stored at -80°C had reduced viability and reduced metabolic activity.

This brings to mind that to keep your cells safe while frozen and thus, viable upon thawing, both temperature and cryoprotectant media are important.