Cryopreservation Best Practices for CAR-T cell Storage
October 25, 2017
For over a year we stored Jurkat CAR-T cells in four different conditions to understand what temperature and cryoprotectant media performs best. The two temperature conditions were -80°C and -190°C and the two medias were a home-brew cocktail of FBS/DMSO 95%/5% and cGMP manufactured CryoStor5. At ISBER earlier this year we presented the results [download poster here].
In summary, the highest performing group was the Jurkat T-cells prepared with CryoStor5 media and stored at -190°C. Their post-thaw viability and metabolic activity after 410 days in storage was nearly identical to the non-shipped control. The group stored at -190°C with home-brew media had reduced viability and both groups stored at -80°C had reduced viability and reduced metabolic activity.
This brings to mind that to keep your cells safe while frozen and thus, viable upon thawing, both temperature and cryoprotectant media are important.